Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Evaluation of cytotoxic and wound healing effect of DMEM extracts of Turkish propolis in MDA-MB-231 cell lines

Meltem Uçar¹ , Orhan Deger²

¹Faculty of Health Science, European University of Lefke, Lefke, Northern Cyprus, TR-10 Mersin; ²Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.

For correspondence:- Meltem Uçar Email: mucar@eul.edu.tr Tel:+903936602000

Accepted: 20 January 2019 Published: 28 February 2019

Citation: Uçar M, Deger O. Evaluation of cytotoxic and wound healing effect of DMEM extracts of Turkish propolis in MDA-MB-231 cell lines. Trop J Pharm Res 2019; 18(2):321-325 doi: 10.4314/tjpr.v18i2.14

© 2019 The authors.
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Abstract

Purpose: To investigate the effect of Dulbecco's Modified Eagle Medium (DMEM) extract of Turkish propolis on proliferation, cytotoxicity and lateral motility in MDA-MB-231 cells.

Methods: The antiproliferative activity of DMEM extracts of propolis was determined colorimetrically in MDA-MB-231 cells using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Cell toxicity and wound healing effects of the prpolis extracts were determined with trypan blue exclusion assay and wound-healing assay, respectively.

Results: The cell number of MDA-MB-231 cells were decreased by the extracts at all concentrations for 72 h. The highest antiproliferative activity of the extract was demonstrated at 10 mg/mL for 24 - 72 h. Moreover, 5 and 0.31 mg/mL of the propolis extract showed significant antiproliferative activity at 72 h of incubation. The extract showed cytotoxic effect to MDA-MB-231 cells at 10 mg/mL. The extract (at a dose of 2.5 mg/mL) during 24 - 72 h did not produce any effect on lateral motility in MDA-MB-231 cells in the wound healing assay.

Conclusion: These results indicate that the DMEM extract of propolis exerts antiproliferative and cytotoxic effects on MDA-MB-231 cells at different concentrations.

Keywords: Propolis, Proliferation, Cytotoxicity, Motility, Breast cancer